Figure 4

H-rev107 suppresses cell migration and invasion. NT2/D1 cells plated in triplicate in 24-well plates were transfected with 500 ng of H-rev107 or control expression vector and then incubated with 500 ng/mL of PGD2 or ethanol vehicle for 24 h. Cells were washed with serum free medium and were seeded in triplicate in polycarbonate membrane inserts in 24-well plates. Lower wells containing 700 μL DMEM supplemented with 20% FBS was served as chemoattractant. Migratory cells were stained after 24 h of incubation (A). Invasive activity was measured using the Matrigel invasion assay after 72 h of incubation in serum containing medium supplemented with PGD2 or ethanol vehicle (B). Representative results of three independent experiments are shown. Ctrl: control; Student’s t-test: *, P < 0.05; ***, P < 0.001.