Figure 5

PTGDS shRNAs prevent H-rev107-mediated enhancement of PGD2, cAMP, and SOX9 levels. NT2/D1 cells were transduced with indicated shRNA for 72 h and then transfected with H-rev107 expression or control vector for 24 h. Levels of PGD2 (A) and cAMP (B) were determined using enzyme immunoassays, and expression of SOX9 was determined by Western blot analysis using anti-SOX9 antibody (C). Representative results from triplicate samples were expressed as means ± SD. Student’s t-test: **, P < 0.01; ***, P < 0.001.