Similar dose-dependence of motor neuron cell death caused by wild type human TDP-43 and mutants with ALS-associated amino acid substitutions

Table 1 Levels of exogenous hTDP-43 proteins relative to the endogenous mTDP-43 in transfected cells*

	Plasmid DNA amount (μg) used for transfection of 10⁶cells
	1.25	2.5	5	10	20
NSC34
WT	0.26	0.56	0.73	0.87	1.1
A315T	0.54	0.77	0.80	1.0	1.3
N390D	0.61	0.86	0.93	1.3	1.5
Neuro2a
WT	0.29	0.49	0.86	1.4	1.7
A315T	0.44	0.65	1.1	1.6	1.9
N390D	0.50	0.89	1.2	1.6	2.2

* The levels of the exogenous hTDP-43 proteins relative to the endogenous mTDP-43 were measured first by Western blot analysis using the Gene Tex antibody recognizing both hTDP-43 and mTDP-43. The ratio of the intensity of the exogenous hTDP-43 to that of the endogenous mTDP-43 at each dose of the transfection was estimated and then calibrated against the average transfection efficiencies of the cells (50% for NSC34 and 70% for Neuro2a cells).

ISSN: 1423-0127