Effects of SOC inhibitors and knockdown of Orai1/STIM1 in BrdU proliferation and cell-cycle progression. (A) ARPE-19 cells were pre-treated by 100 μM 2-APB or 20 μM SKF96365 for 30 min. Cells were applied by 25 ng/mL EGF for 24 h. BrdU incorporation assay was used. (B) ARPE-19 cells were transfected with Orai1 and STIM1 siRNA. The 25 ng/mL EGF-induced BrdU incorporation was detected. (C) The cell-cycle assessed by flow cytometry showed that 25 ng/mL EGF incubation promoted the cell-cycle progression by increasing G2/M percentage, and the 20 μM SKF96365 arrested the cell-cycle in the G0/G1 phase.