EGF induced ARPE-19 proliferation and migration via MEK/ERK 1/2 pathway, independent of SOC channel pathway. (A) ARPE-19 cells were pre-treated with 20 μM PD98059 or 10 μM U0126 for 30 min and were stimulated by 25 ng/mL EGF for 48 h. WST-1 assay was performed. (B) Both 20 μM PD98059 and 10 μM U0126 efficiently reduced the ERK 1/2 phosphorylation in ARPE-19 cells evoked by 25 ng/mL EGF. (C) ARPE-19 cells were applied with 20 μM PD98059 or 10 μM U0126 for 30 min followed by 25 ng/mL EGF for 24 h and 48 h. Migration assay was performed. (D) Quantitative analysis of cell migration of Figure 7C. (E) Cells were applied by 100 μM 2-APB and 20 μM SKF96365, EGF-induced ERK 1/2 phosphorylation was determined by Western blots.