Gene copy numbers of the samples. (A) A scattergram of the EGFR or PIK3CA results are shown. Each dot represents the specific GCN of the individual specimen analyzed using real-time PCR and the C-2∆tt method. Dots above the upper dotted line have copy number of more than 3. Dots above the lower dashed line have copy number of more than 2. (B) An association study of EGFR expression and GCN amplification is shown by the bar chart. The empty and solid bars represent negative or positive GCN amplification. The P level was assessed through Chi-Square analysis.