Figure 4
Determination of critical residues in P6 and P19 peptides. A) Alanine substitution scan of the peptides. Biotinylated peptides (30 μg/ml) were allowed to bind immobilized gp120BaL (8 μg/ml). P6/34 means that Asp34 in P6 was substituted by alanine. It was same to other peptides, such as P6/35, P19/96, P19/101 and P19/96/101; P1 was used as a negative control; B) The structural view of P6 and P19 in SRCR1 model. The P6 was colored in cyan and P19 was colored in purple. Asp34, Asp35, Asn96 and Glu101 residues were labeled on the regions of P6 and P19.