MFN2 overexpression improved insulin sensitivity in rats. Rats were fed with high-fat diets for 8 weeks, and then were infected with Ad-Mfn2 (109 v.p./kg body weight) or empty Ad adenoviruses or PBS control for 3 weeks. MFN2 overexpression in skeletal muscle of rats was confirmed by quantitative RT-PCR (A) and Western blot analyses (B, C). The levels of blood glucose (D), plasma insulin (E) and insulin sensitivity (F) were assayed. *P < 0.05, **P < 0.01, compared with Ad, n = 6.