Figure 4

Acute effect of tBHP on SOCE. Fura-2-loaded CHO-M3 cells were stimulated with 10 μM carbamylcholine at the point indicated by the labeled arrow. tBHP (20 mM) was added at the 120 sec time point, as indicated by the arrow labeled “tBHP”. Under these assay conditions, the addition of direct inhibitors of SOCE channels, such as honokiol [21], 2-aminoethoxydiphenylborane, 1-(5-chloronaphthalenesulfonyl)homo piperazine hydrochloride (ML 9), and ZnO nanoparticles, cause an immediate reduction of SOCE; data from a typical experiment with 30 μM honokiol are presented in the insert for comparison purposes.