Figure 4

Effects of human apoE on neurite outgrowth in OE cultures. Two-days old cultures from apoE KO mice were grown in medium alone, and in medium containing 5 μg/ml of human apoE2, human apoE3, or human apoE4. Medium was changed every two days, and apoE re-added. Cultures were fixed at 8 DIV, and neurite outgrowth (* p < 0.05) was measured in tubulin III immunopositive neurons as described under Methods.