Figure 5

Effects of blocking lipoprotein receptor, LRP, on differential effects of human apoE on neurite outgrowth in OE cultures. Two-days old cultures from apoE KO mice were incubated for 1 h in medium alone or in medium containing receptor associated protein (RAP) (5 μg/ml) or lactoferrin (LAC, 10 μg/ml). Following incubation with RAP or LAC, human apoE isoform (5 μg/ml) was added to the medium and incubation was continued for a total of 8 days. Medium was changed every two days, and test reagents re-added. Cultures were fixed, and neurite outgrowth (* p < 0.05) was measured in tubulin III immunopositive neurons as described under Methods.