Effect of in vivo model of aorta-caval shunt (AV shunt) on aortic myocardin protein levels. (A) Western blots for myocardin in rat aorta after short-term induction of AV shunt. (B) Quantitative analysis of myocardin protein levels. The values have been normalized to GAPDH measurement and then expressed as a ratio of normalized values to myocardin protein in sham. (C and D) Treatment with losartan (10 mg/kg/day) throughout day 1 to day 5 after performing aorta-caval shunt signigicantly suppressed myocardin protein expression from day 1 to day 3. *P < 0.01 vs. control. #P < 0.05 vs. 24 h (n = 5).