Figure 2
From: Promotion of wound healing using adipose-derived stem cells in radiation ulcer of a rat model
Effects of passage number on mRNA expression and secretion of VEGF and HGF. Passage numbers were shown as P1, P2, etc. (A- D) Total mRNAs were extracted from three different rat ASCs and reverse transcribed into cDNA. The VEGF (A) and HGF (B) mRNA expression levels were analyzed using real-time PCR analysis. Values were normalized to the level of GAPDH mRNA and expressed relative to normalized values of P1. Secretion of VEGF (C) and HGF (D) by rat ASCs (n = 3) cultured over 72 hours was measured by ELISA. The data represent the mean ± SEM. *p < 0.05, **p < 0.01.