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Figure 5 | Journal of Biomedical Science

Figure 5

From: Promotion of wound healing using adipose-derived stem cells in radiation ulcer of a rat model

Figure 5

Analysis of angiogenesis and blood vessel density in ASC-treated tissue. (A-C) After three weeks of treatment, the tissues of the wound edge were subjected to histological examination. Compared with the control group (A), the capillary density in the dermis was clearly increased in the ASC-treated group (B). Arrows indicate vessel formation and proliferation sites containing erythrocytes in each wound. E: epidermis; M: muscle layer; U: ulcer. Bar: 500 μm. (C) Tissue lysates from re-epithelialized skin tissue of control and ASC-treated groups were analyzed with immunoblot analysis against CD31 and HuR (a loading control). (D-F) Three weeks after ASC treatment, ulcerated tissues were removed and processed into 8 μm frozen sections. Slides were stained with CD31 (green) and DAPI, a fluorescent nuclear stain (blue). Bar: 50 μm. Representative images of the control (D) and ASC (E) group are shown. (F) The blood vessel density was evaluated in the control and ASC-treated groups. The data represent the mean ± SEM of eight rats. *p < 0.01.

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