Figure 3

Effects of FGF-2, antioxidants, and hypoxia on the cell cycle profiles of ADMSCs. (A) The percentage of cells in each phase of the cell cycle at day 3. (B) Relative levels of p21 and p27 mRNAs in ADMSCs from the various culture conditions. Expression of β-actin served as the internal control. Expression values were normalized to the corresponding values measured for cells cultured under the Im condition. (C) Western blot analysis showed that CDK4 increased, but p21 and p27 decreased, in cells cultured under ImF-H or ImF-A conditions. (D) Schematic diagram of antioxidant effects on cell cycle progression. The bars represent the mean value ± SD (n = 3). * P < 0.05. ** P < 0.01. *** P < 0.005.