Figure 6

Differentiation potential of ADMSCs after 6 days under the four culture conditions. (A) Photomicrographs showing alkaline phosphatase activity of ADMSCs after 14 days osteo-differentiation. Bar = 500 μm. (B) Photomicrographs of lipid spheres in ADMSCs after 7 days adipo-differentiation. Lipid spheres were visualized by Oil Red O staining. Bar = 500 μm. (C) Photomicrographs of sulfated proteoglycan-rich matrix (blue color) in ADMSCs after 14 days chondro -differentiation. (D) Quantification of osteogenic mRNA in ADMSCs after 14 days osteo-differentiation. (E) Quantification of adipogenic mRNA in ADMSCs after 7 days adipo-differentiation. (F) Quantification of chondrogenic mRNA in ADMSCs after 7 days adipo-differentiation. Expression of β-actin served as the internal control. Each bar represents the mean value ± SD (n = 3). * P < 0.05. ** P < 0.01. *** P < 0.005.