Figure 1

Microglia of SR-A knockout mice internalizes less oAβ and AcLDL. A and B, Primary microglia isolated from wild-type (WT) and SR-A knockout mice were incubated with fluorescent oAβ and AcLDL. Representative confocal images and quantification of relative fluorescence intensities of internalized oAβ and AcLDL showed that less internalization of oAβ and AcLDL by microglia of SR-A knockout mice. Scale bar, 20 μm. More than 60 cells were analyzed. Bars indicate mean ± SEM of three independent experiments (*p < 0.05). C, Primary microglia, human blood-derived macrophages (HMϕ), and J774 cells were incubated with FAM-oAβ and immunostained with anti-SR-A antibody. Representative confocal images showed that oAβ was highly co-localized with SR-A in endocytic vesicles. The experiments were repeated at least three times. Scale bar, 10 μm.