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Figure 6 | Journal of Biomedical Science

Figure 6

From: Cysteine-rich domain of scavenger receptor AI modulates the efficacy of surface targeting and mediates oligomeric Aβ internalization

Figure 6

Both SRCR and collagenous domains of SR-AI are oAβ- and AcLDL-binding domains. A and B, Western blot analysis of total cell lysates and avidin pull-down of biotinylated lysates after PNGase F cleavage. C, Relative levels of surface-targeted SR-AI variants were quantified by densitometry. D, Western blot analysis of total lysates of transfected cells after PNGase F or Endo H cleavage. The experiments were repeated at least three times. E and F, Relative fluorescence intensities of internalized oAβ and AcLDL for more than 100 SR-AI variant-positive cells were quantified using MetaMorph software. Bars indicate mean ± SEM of three independent experiments. Experimental groups labeled with different letters were significantly different from each other (p < 0.05).

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