Figure 2

Effect of LPS, IFN-γ, and IFN-γ + fAβ on nitric oxide production in BV-2 cells. BV-2 cells were treated with indicated concentrations of LPS (a, b) or vehicle, 0.1 and 0.2 ng/ml IFN-γ alone, vehicle or FN-γ + 1 μM fAβ (c, d), for 24 h. (a, c) After treatment, cultured medium was collected and the nitrite content was determined. (b, d) The cell viability of the treated cells was assayed by MTT reduction. Results are means ± S.D. from three independent experiments. Significant differences between cells treated with vehicle (Veh) and LPS or IFN-γ is indicated by *, p < 0.05, ***, p < 0.001. Significant differences between the cells treated with IFN-γ alone and IFN-γ combined with fAβ are indicated by ###, p < 0.001.