Figure 6

CC-EW and CCB inhibit oAβ-induced morphological alteration of microglia and reduce the Aβ level. Glia were treated with vehicle (A), 20 μg/ml CC-EW (AC20), 100 μg/ml CC-EW (AC100), or 20 μM CCB (ACB20) for 2 h, and then were treated with 5 μM oAβ for 24 h. (a) Cells were fixed and subjected to immuno-staining by anti-Iba1 antibody (red), anti-GFAP antibody and anti-Aβ antibody (green). Nuclei were stained using Hoechst 33258 (blue). Results were repeated for three times, and represent photograph are shown. (b) Microglial form factor were calculated as describe in the Method section. Results are means ± S.D. from three independent experiments and total nine cells of each group were analyzed. (c) The number of Aβ-immunoreactive deposit per field (250 μm × 250 μm) of view was calculated using Metamorph software, and presented in percentage of the cells treated with Aβ alone. (d) The conditioned medium was collected and subjected to Aβ1-42 ELISA assay. The data is presented in percentage of the cells treated with Aβ alone. Results are means ± S.D. from three independent experiments. Significant differences between oAβ group and other treated group are indicated by **, p < 0.01; ***, p < 0.001.