CIP4 is important for neonatal rat ventricular myocyte hypertrophy. A. Myocytes were transfected with control or CIP4 siRNA oligonucleotides and cultured ± 10% fetal bovine serum (FBS), 10 μM PE, or 1000 U/mL LIF. After treatment for 2 days, the myocytes were stained for α-actinin (green), ANF (red) and Hoechst (blue); bar = 20 μm. B. Cross-section area of myocytes in A. C. Fraction of myocytes expressing ANF in A. n = 5 for B and C. ANOVA (two-factor with replication): p-value (CIP4 siRNA vs. control siRNA) = 6 × 10-5(B) and = 0.046 (C); p-value (between culture conditions) < 10-7 for both B and C. Post-hoc testing: *p-values vs. control siRNA-transfected myocytes treated with the same agonist; †p-values vs. no drug control. D. [3H]leucine incorporation. n = 2. *p-value vs. PE-treated, control siRNA-transfected myocytes.