Dengue-2 virus infection induces LC3 puncta formation and increases LC3 II expression in the mouse brain tissue. As in Figure 1, DV2 was injected into the six-day-old suckling mice. (A) At day 5 p.i., the brain tissues were sectioned and the expression of DV2-NS1 and LC3 in the mouse brain tissue was determined with anti-DV NS1 and anti-LC3 antibodies by IFA staining under the confocal microscope (Olympus FluoView 1000). DAPI was used to stain the nucleus of the cell. Arrow points indicate the colocalization of NS1 and LC3. (B) The brain tissues of the mice used in (A) were harvested and total protein lysate was collected at various times p.i. and analyzed by Western blotting to measure the expression of DV2-NS1 and LC3 with the anti-DV NS1 and anti-LC3 antibodies. The numbers under each band are the quantification of LC3II band intensity after normalization with β-actin.