DV2 infection of mice brain induces amphisome formation. The same treatment used in the above figures was conducted. The expression of NS1, the endosome marker MPR, and the autophagosome marker LC3 protein in the sections of the mice brain tissue was shown by IFA staining under a confocal microscope using specific antibodies followed by secondary fluorescent conjugation. The DAPI was used to indicate the nucleus of the cells. (A) colocalization of LC3 (green) and MPR (red); (B) colocalization of NS1 (red) and MPR (green). Arrow points indicate colocalization of LC3 and MPR or NS1 and MPR.