Manipulation of autophagy by 3-MA or rapamycin affects the clinical score and survival rate of DV2-infected suckling mice. The six-day-old ICR suckling mice were divided into three groups. The same treatment as in Figure 6 was conducted. (A) The clinical score in (A) for 3-MA treatment and in (B) for rapamacin treatment, as well as survival rate in (C) for 3-MA treatment and in (D) for rapamycin were monitored daily after the treatments. The criteria of the clinical score are described in Figure 1.