Figure 1
From: Active RHOA favors retention of human hematopoietic stem/progenitor cells in their niche
RHOA endogenous and exogenous expression. (A) Real-time PCR analysis to determine the mRNA levels of RHOA in lineage depleted cord blood hematopoietic sub populations CD34+CD38- and CD34+CD38+ showed that RHOA was expressed in both the populations and it was significantly higher in CD34+CD38- stem cell population. (B) Replication incompetent lentiviral vectors expressing RHOAV14 or RHOAN19 with SFFV (Spleen focus forming virus) promoter with EGFP (enhanced Green fluorescent protein) driven by IRES (Internal Ribosome Entry Site).