Figure 3
From: Active RHOA favors retention of human hematopoietic stem/progenitor cells in their niche
Expression of CD62L and cell cycle. (A) Flow cytometry analysis of CD62L expression in control, RHOAV14 and RHOAN19 HSPCs. Plots are a representative of at least 3 independent experiments. (B) RHOA transduced HSPCs were analyzed for their cell surface CD62L expression. Values are mean ± SD, n = 3 independent experiments. (C) RHOA modified HSPCs were cultured in liquid culture in the presence of cytokines and at day 7 were analyzed for their cell cycle profile using Ki-67 and DAPI. Plots are a representation of at least 3 independent experiments. (D) Graph representing the G0 percentage of control, RHOAV14 and RHOAN19 HPC/HSCs after 7 days in liquid culture. Values are Mean ± SD, n = 3 independent experiments.