Myo6 deficiency induces endosome accumulation in epithelial cells. A-B, the immune blots indicate the levels of myo6 (A) or EEA1 (B; a marker of endosome) in epithelial cells. The bars below he blots indicate the summarized integrated density of the blots. C, the confocal images show the EEA1 staining (in red) in epithelial cells. D, the bars indicate the average number of endosomes in epithelial cells, which was averaged from 30 cells. Naïve: Naïve epithelial cells. shRNA: The epithelial cells were treated with shRNA of myo6. cshRNA: The epithelial cells were treated with control shRNA. The data in bar graphs were presented as mean ± SD. *, p < 0.01, compared with the naïve group. The data represent 3 separate experiments.