Figure 3

Assessment of epithelial monolayer permeability. A, the bars indicate the levels of OVA in basal chambers (assessed by ELISA). B, the immune blots indicate OVA in basal chambers (assessed by Western blotting). C, the bars indicate the levels of ⁵¹Cr-EDTA in basal chambers (assessed by a γ counter). D, the bars indicate the TER of the epithelial monolayers recorded at o h and 24 h respectively. The data in bar graphs were presented as mean ± SD. *, p < 0.05, compared with group 12 h (C) or naïve group (D). Naïve: Naïve epithelial cells. shRNA: The epithelial cells were treated with shRNA of myo6. cshRNA: The epithelial cells were treated with control shRNA. The data represent 3 separate experiments.