Actin cytoskeleton rearrangement during MSC differentiation. (A) MSC were grown in media containing osteogenic or adipogenic inducers for 24 hours, 3 days, 7 days and 14 days or left uninduced (0 hr) and F-actin was visualised by staining with phalloidin-TRITC. Photomicrographs are representative images from 3 independent experiments. (B) Flow cytometric analysis of uninduced (CONTROL) or MSC induced with osteogenic or adipogenic media for 24 hour and stained with phalloidin-TRITC. X-axis represents the F-actin fluorescence intensity, representative graphs are shown. Unstained cells were used to obtain the auto fluorescence levels during flow cytometry.