Figure 2From: Inhibition of actin polymerization decreases osteogeneic differentiation of mesenchymal stem cells through p38 MAPK pathwayActin cytoskeleton rearrangement during MSC differentiation. (A) MSC were grown in media containing osteogenic or adipogenic inducers for 24 hours, 3 days, 7 days and 14 days or left uninduced (0 hr) and F-actin was visualised by staining with phalloidin-TRITC. Photomicrographs are representative images from 3 independent experiments. (B) Flow cytometric analysis of uninduced (CONTROL) or MSC induced with osteogenic or adipogenic media for 24 hour and stained with phalloidin-TRITC. X-axis represents the F-actin fluorescence intensity, representative graphs are shown. Unstained cells were used to obtain the auto fluorescence levels during flow cytometry.Back to article page