Effect of CYD treatment on adipogenic differentiation. (A) Percentage of MSC differentiated into adipocytes for 7 days without (CONTROL) or with CYD treatment (+CYD) was determined by ORO staining where CYD treatment was done for the initial 3 days (3D) during the 7 days induction period. F-actin staining with phalloidin-TRITC for CYD treated MSC is shown in the inset. (B) F-actin and ORO staining in MSC induced to differentiate into adipocytes without (CONTROL) or with CYD treatment (+CYD) for 7 or 14 days. Representative photomicrographs are shown. (C) Fold increase in ORO positive cells in MSC were induced to undergo adipogenesis without (CONTROL) or with CYD (+CYD) for the indicated time periods: (D) ADIPOQ and PPARG mRNA levels in untreated (CONTROL) or CYD treated (+CYD) MSC induced to undergo adipogenesis for the indicated time periods. Values are mean ± SD, n = 3-4. * p < 0.05, ** P < 0.005, *** p < 0.0005, ORO stands for Oil red O.