Figure 6

p38MAPK and CD49E expression changes during MSC differentiation. MSC were differentiated into osteocytes (OS) or adipocytes (AD) in the absence (UNTR) or presence of CYD (+CYD) for 3 days and the phosphorylated levels of p38 MAPK (A) and ERK1/2 MAPK (C) was determined by flow cytometry. Y-axis in figure A and C shows the relative phosphorylated levels of p38 MAPK or ERK1/2 MAPK compared to undifferentiated untreated control cells (CONTROL). B. Representative flow cytometric histrograms of MSC differentiated into osteocytes (OS) or adipocytes (AD) which were left untreated (UNTR) or treated with CYD (+CYD) stained for phosphorylated form of p38MAPK. Red line is the isotype control and blue line is the sample. D. Flow cytometric analysis of CD49E surface expression in MSC differentiated into osteocytes (OS) or adipocytes (AD) which were left untreated (UNTR) or treated with CYD (+CYD) for 14 days. Values are mean ± SD, n = 3, *p < 0.05, **p < 0.005, *** p < 0.0005.