A20 levels in colon cancer tissue. Colon cancer tissue was collected from 88 patients with colon cancer. Control colon tissue was collected from 10 patients with IBS (irritable bowel syndrome). A, the bars indicate the levels of A20 or p53 mRNA (by qRT-PCR). B and C, the immune blots indicate the protein levels of A20 (B) and p53 (C). The bars below the immune blots indicate the integrated density of the immune blots. The samples from different patients were processed individually (samples were processed in respect sample tubes in qRT-PCR and respect sample wells in Western blotting). D, the dot plots indicate the correlation between the polyp history and A20 levels in the polyp tissue (each dot represents one sample; some dots are overlapped). E, the representative confocal images indicate the A20 staining (in green; the red color indicates the nuclearing staining) in cancerous tissue (E1) and IBS colon tissue (E2). E3 is an isotype control. *, p < 0.05, compared with the control group. Samples from individual patients were processed separately.