Figure 4
From: Tranilast enhances the anti-tumor effects of tamoxifen on human breast cancer cells in vitro
MCF-7 (A) and MDA-MB-231 (B) cells were stained by AO/EB and observed under fluorescence microscope: a control group; b in the presence of 2 μM TAM; c in the presence of 200 μM tranilast; d in the presence of in combination both. Green live cells show normal morphology; green early apoptotic cells show nuclear margination and chromatin condensation. Late orange apoptotic cells showed fragmented chromatin and apoptotic bodies. Scale bar: 10 μm. L = live cells, A = early apoptotic cells, LA = late apoptotic cells, N = necrotic cells.