Figure 7
From: Tranilast enhances the anti-tumor effects of tamoxifen on human breast cancer cells in vitro
TAM and tranilast regulate TGF-β ligands and receptors mRNA expression in MCF-7 and MDA-MB-231 cells. Quantitative RT-PCR analysis in MCF-7 cells showed that TAM and tranilast as a single or in combination effectively decreased the expression of TGF-β1, 2, 3 and TβRI, II mRNA. However, MCF-7 cells are stimulated by TAM and/or tranilast to increase TβRIII expression slightly. The real-time RT-PCR results were normalized against the internal control GAPDH and are expressed as a percentage of control cells. All gene expression values were obtained in single experiments performed in triplicates. Different to control *p < 0.05, **p < 0.01, ***p < 0.001.