Figure 4

Immunohistochemical (IHC) staining of the innate inflammatory cells in infarct area (IA) and peri-IA at day 21 after AMI induction. Upper panel: A to E) Illustration of IHC staining of CD14+ cells (yellow arrows) in infarct area (IA) and peri-IA of left ventricle. F) Quantification of number of CD14+ cells. At IA, * vs. other bars with different symbols, p<0.0001. At peri-IA, * vs. other bars with different symbols, p<0.01. (n=6 for each group). Lower panel: A to E) Illustration of IHC staining of inducible nitric oxide synthase (iNOS) + cells (yellow arrows) in IA and peri-IA of left ventricle. F) Quantification of iNOS+ cells. At IA, * vs. other bars with different symbols, p<0.0001. At peri-IA, * vs. other bars with different symbols, p<0.001. All statistical analyses using one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡ or *, §, ¶) indicate significance (at 0.05 level). HPF = high-power field; AMI = acute myocardial infarction; Tac = tacrolimus. (n=6 for each group).