Figure 5

Protein expressions of innate inflammatory biomarkers of TNF-α, NF-κB, and IL-12 by day 21 after AMI induction. A) The protein expressions tumor necrotic factor (TNF)-α in infarct area (IA). * vs. other bars with different symbols, p<0.001. B) The protein expression of TNF-α in per-IA. * vs. other bars with different symbols, p<0.01. C) The ratio of protein expression of phosphorylated (p) nuclear factor (NF)-κB/total NF-κB in IA. * vs. other bars with different symbols, p<0.01. D) The ratio of protein expression of p-NF-κB/total NF-κB in peri-IA. * vs. other bars with different symbols, p<0.05. E) Protein expression of interleukin (IL)-12 in IA. * vs. other bars with different symbols, p<0.001. F) Protein expression of IL-12 in peri-IA. * vs. other bars with different symbols, p<0.0001. All statistical analyses using one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡) indicate significance (at 0.05 level). NC = normal control; AMI = acute myocardial infarction; Tac = tacrolimus. (n=6 for each group).