Immunofluoroscopic findings of γ-H2AX+ cells in peri-infarct area (IA) and non-IA by day 21 after AMI induction. A to E) The illustration of immunofluorescent (IF) stain of γ-H2AX+ cells in peri-IA and non-IA. F) Quantification of number of γ-H2AX+ cells in peri-IA and non-IA. At peri-IA, * vs. other bars with different symbols, p<0.0001. At non-IA, * vs. other bars with different symbols, p<0.001. All statistical analyses using one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡ or *, §, ¶) indicate significance (at 0.05 level). HPF = high-power field; NC = normal control; AMI = acute myocardial infarction; Tac = tacrolimus. (n=6 for each group).