Immunofluoroscopic findings of connexin43 expression in peri-infarct area (IA) and non-IA by day 21 after AMI. A to E) The illustration of immunofluorescent (IF) stain of connexin43 (Cx43) expression in peri-IA and non-IA. F) Quantification of CX43 distribution (area) in per-IA and non-IA. At peri-IA, * vs. other bars with different symbols, p<0.0001. At non-IA, * vs. other bars with different symbols, p<0.001. All statistical analyses using one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡ or *, §, ¶) indicate significance (at 0.05 level). HPF = high-power field; AMI = acute myocardial infarction; Tac = tacrolimus. (n=6 for each group).