Figure 4

Probing ROCKI and ROCKII activation in cells. Normal mouse embryonic fibroblasts (MEFs) were serum starved for 24 hr and then stimulated with 10 μM of nocodazole in the presence or absence of 10 μM of Y27632. Cells were harvested at the indicated time for Western blotting with antibodies as indicated. The fold of relative protein phosphorylation was measured and showed.