Figure 3

Effects of WEL on LPS-induced NO, PGE2 and TNF-α production in RAW 264.7 macrophages. (A) Cells were treated with the indicated concentrations of WEL (0, 0.1, 1, or 10 μ M) or 100 μM L-NAME for 12 h, respectively, and then incubated with LPS (1 μg/ml) for 20 h. 100 μM L-NAME group was set as positive control. The nitrite production was measured by the Griess reaction. (B, C) Cells were treated with the indicated concentrations of WEL 12 h or 0.1 DX (0.1 μM) for 1 h, respectively, and then incubated with LPS (1 μg/ml) treatment for 20 h. The PGE2 and TNF-α concentration were determined by ELISA kit. The values were presented as mean ± SEM. of three independent experiments. n = 6 per experiment. ^*p < 0. 05 vs. LPS group and ^**p < 0.01 vs. LPS group.^#p < 0. 05 vs. none LPS control group. ^##p < 0. 01 vs. none LPS control group.