Figure 6

Effects of the WEL on the activation of ERK1/2 (A), JNK (B) and p38 (C) in LPS-stimulated cells. Cells were pre-incubated with WEL for 12 h, and then incubated with LPS (1 μg/mL) for 30 min. The total protein lysate was subjected to Western blotting analysis. The ratios of immunointensity of p-ERK1/2, p-JNK and p-p38 were calculated, respectively. Total ERK1/2, JNK and p38 (t-ERK1/2, t-JNK and t-p38) were used as a control of the protein amount in the same samples. A representative blot of each experiment is shown with the densitometric anaylsis corresponding to the mean ± SEM. of three independent experiments. n = 6 per experiment. *p < 0.05 vs. LPS group and **p < 0.01 vs. LPS group. ^#p < 0.05 vs. none LPS control group. ^##p < 0.01 vs. none LPS control group.