EGCg-induced fluorescence changes in EGFP-expressing H9c2 cells and molecular identification on the EGFP-conjugated protein complex. (a) Fluorescence spectra showing the dose effect of EGCg on EGFP fluorescence. (b) Normalized EGFP fluorescence in the absence or presence of 0.1% Triton X-100. Fluorescence spectroscopy was performed as described in the Materials and Methods. The fluorescence emitted at 507 nm measured in the absence of EGCg was used to normalize the fluorescence changes caused by EGCg titrations. Each value is the mean of six measurements. (c) The EGFP co-precipitated proteins were separated by one-dimensional SDS-PAGE or (d) two-dimensional electrophoresis (2-DE), followed by proteomics acquiring MALDI-MS spectra. A co-immunoprecipitation assay reveals molecular identities by immunoblotting (IB) of the protein complexes (i.e., LR, β-actin, GAPDH, Cav-1 and -3) formed with EGFP in these cells.