The caspase 3 activation in the hippocampus of the KA-icv-injected mice. CD-1 mice received KA-icv-injections and were sacrificed at day 1, 3, 5, and 7 post KA-injection (KA-d1, KA-d3, KA-d5, and KA-d7). For control, the mice were sacrificed at day 7 post vehicle-injection (veh). The caspase 3 activation and the reactive astrocytes in the ipsilateral side of CA1, CA3, and DG was examined by immunostaining with anti-active caspase 3 (aCas3, red) and anti-GFAP (green) antibodies. Cell nuclei were stained with Hoechst 33258 (blue). Panel (a) shows the representative photograph of the partial co-localization of active caspase 3 (aCas3) and GFAP in the ipsilateral side of CA1and DG at day 5 and 7 post KA-injection. The location of pyramidal layer (Py), striatum radiatum (SR). Molecular layer (Mo), and Hilus (Hi) are indicated in left lane of the panels. Arrows indicate the representative colocalization of aCas3-IR with astrocytes and radial glial cells in CA1 and DG, respectively. Arrow heads indicate the activation of capsease 3 in pyramidal neurons in CA1. The IR of non-astroglial (b), astroglial (c) active caspase 3 in the ipsilateral side of CA1 (white columns), CA3 (grey columns), and DG (black columns) were calculated using MetaMorph software. The data are presented as the percentage relative to the vehicle injection. The results are the mean ± S.D. from 8 images. The data are the percentages relative to the vehicle injection. Significant differences between vehicle injection and KA injection are indicated by ***, P < 0.001.