The alteration of the neuroplasticity-related proteins in the hippocampus of KA-icv-injected mice is caspase 3 dependent. CD-1 mice received KA-icv-injections and were sacrificed at day 1, 3, 5, and 7 post KA-injection. For control, the mice were sacrificed at day 7 post vehicle-injection (veh). Hippocampus The homogenate and PSD domain of hippocampus were prepared. The GluR2, GluR1, NR-1 and SAP-102 of PSD domain (a) and CN-A of total hippocampal homogenate (b) were analyzed by immunoblotting. (c) CD-1 mice received KA-icv-injections alone or co-injection of KA and caspase 3 inhibitor (Cas3I) or vehicle (veh), and were sacrificed at day 3 or 7 post KA-injection (KA-d3 and KA-d7). Hippocampal lysates were analyzed by immunoblotting. PSD-95 and β-actin were used as internal standard for (a) and (b, c), respectively. The top part is the representative immunoblots. The bottom part in (a) is the ratio of proteins/PSD-95 relative to the ratio of the control (veh). The bottom part in (b) and (c) show the percentage of the level of 65 kD (open circles) and 36 kD (closed circles) relative to the level of 65 kD in the control (veh). Results are mean ± S.D. from five independent experiments. Significant differences between the control (veh) and KA injection in (a, b) are indicated by *, P < 0.001. Significant differences between the mice injected with and without caspase 3 inhibitor in (c) are indicated by *, P < 0.001.