DNA transposon-based gene vehicles - scenes from an evolutionary drive

Table 1 DNA transposon vectors and their main characteristics

Transposon	Transposon family	Target sequence	Integration preference	Cargo capacity	Footprint	Overproduction inhibition	Hyperactive transposase	References
Sleeping Beauty	Tc1/mariner	TA	Fairly random (31-39% into genes)	~ 10 kb	C(A/T)GTA	Yes	SB100X	[50–54]
Frog Prince	Tc1/mariner	TA	ND	ND	C(A/T)GTA	ND	None	[30]
Hsmar1	Tc1/mariner	TA	Fairly random (44% into genes)	ND	T(T/A)A	Yes	None	[34, 55, 56]
Himar1	Tc1/mariner	TA	Fairly random	4 kb	ACTA	Yes	C9	[31, 57–59]
Passport	Tc1/mariner	TA	Transcriptional units (63% into genes)	ND	ND	Yes	None	[33]
Tol2	hAT	8 bp random sequence	Transcriptional units (39-48% into genes)	> 11 kb	8 bp random sequence	Limited	None	[60–62]
TcBuster	hAT	8 bp random sequence	Transcriptional units	ND	8 bp random sequence	ND	TcBuster_COV₅₉₆A	[36, 37]
PiggyBac	PiggyBac	TTAA	Transcriptional units (47-67% into genes)	100 kb	None	Conflicting reports	7pB, hyPBase	[27–29, 63, 64]
PiggyBat	PiggyBac	TTAA	Transcriptional units	ND	ND	ND	None	[35]

ISSN: 1423-0127