Representative immunofluorescent analyses for Cofilin identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin. H9C2 cells in different treatment conditions were fixed and incubated with anti-Cofilin-1 antibody (Red) and stained with Phalloidin (Green). Nucleus was stained with DAPI (Blue). Each set of three fields was taken using the same exposure, and images are representative of three different fields. In this validation experiment, H9C2 cells were left untreated, treated with 0.45 µM doxorubicin for 24 h or pretreated with 100 µM quercetin for 4 h followed by 0.45 µM doxorubicin for further 24 h.