Morphological analysis of C. albicans strains capable of Dox-inducing the expression of assorted CaCDC4 domains. Cells were grown initially in SD medium without Met/Cys to saturation and were diluted to the same initial concentration. Cells were grown exponentially in SD in the absence of 2.5 mM Met/Cys, with or without 50 μg/ml Dox (-Met/Cys + Dox or -Met/Cys), or in the presence of 2.5 mM Met/Cys, with or without 50 μg/ml Dox (+Met/Cys + Dox or + Met/Cys). (A) The Dox-inducibly expressing assorted Ca Cdc4 protein domains under CaMET3-CaCDC4 repressed conditions was verified by Western blotting with polyclonal antibody to FLAG. The non-specific signals between 72 and 55 kDa, and between 55 and 40 kDa are served as a loading controls. (B) The images were visualized and recorded with a Nikon 50i microscope at 400× magnification. The arrow in white indicates filamentous cells. Bars represent 10 μm. The designations of strains are the same as in Table 1.