Analysis of cell flocculation by low-speed centrifugation. Cultures of the indicated strains were grown in SD medium with histidine, arginine, uridine for 2 days before diluting into the SD medium to an initial OD600 = 0.1 with addition of 2.5 mM Met/Cys to repress the expression of CaMET3 p-driven CaCDC4 and 40 μg/ml Dox to induce the expression of Ca Cdc4 domains tested for 48 hrs to OD600 ≈ 1.6. Cultures were photographed before and after centrifugation. (A). A representative of the cultures. Upper panel: two-day culture. Bottom panel: cultures being spun down with 500 rpm for 1 minute. (B). Quantitative results. Data are represented as means with standard deviation from three independent experiments, each sample was in duplication. The data from JSCA0022 were compared with those of other strains. **: P < 0.01. The designations of strains are the same as in Table 1.