Dissection of the Candida albicans Cdc4 protein reveals the involvement of domains in morphogenesis and cell flocculation

Table 2 Oligonucleotides used in this study

Name	Sequence^a
CaCDC4 XhoI F	GAACTCGAGA TGGATAAGAAATCAAAG
CaCDC4 XhoI R	GAACTCGAG CTGTAAAAGTGGTTGACT
CaCDC4 SalI	TAGCGTCGAC ATGGATAAGAAATCAAAGC
CaCDC4 BglII	TCGAGATCT TCACTGTAAAAGTGGTTGAC
CaURA3-dpl200 BamHI	AATGGATCC CCAGATATTGAAGGTAAAAGG
CaURA3-dpl200 XhoI	ATTCTCGAG CTAGAAGGACCACCTTTGAT
TET25M KpnI	CAAGGTACC GAACCATCGTGAGTGTAA
TET25M BamHI	GAAGGATCC CGACATTTTATGATGGAA
CaCDC4-6HF SalI	GCGTGTCGAC GTCATGGATAAGAAATCAAAGCTA
CaCDC4-6HF^b BglII	TCGAGATCT ttatttatcatcatcatctttataatcACCACCgtggtggtggtggtggtgCTCGAG CGGCCGC TGTAAAAGTGGTTGACTGAAATC
CaCDC4 ΔN AatII	AATAGACGTC CTTATGCCCTCATGTGACGAC
CaCDC4 ΔN XhoI	ATCCTCGAG CTGTAAAAGTGGTTGACTGA
CaCDC4 F-box AatII	AAGCGACGTC ATGAGCAATGAACCTACT
CaCDC4 F-box XhoI	GCCACTCGAG CCACCTATTGACAATTAT
CaCDC4 WD40 AatII	GCTAGACGTC ATGGATCCAAAGTTCAAAC
CaCDC4-URA3-F	ATGGATAAGAAATCAAAGCTATTCAAATATCCTTTGAGCGAGGAGACGGCTAAATTTGAGGTTTTCCCAGTCACGACGTT
CaCDC4-URA3-R	TCACTGTAAAAGTGGTTGACTGAAATCTAGAATCTCAATAAACGTTTCACCTTCATCTTCTGTGGAATTGTGAGCGGATA
CaADH1_probe_F	GGAGTATTGGCATTGTTGGG
CaADH1_probe_R	AAGCTTGCTTGCATGACGAG
CaCDC4_probe_F	GGTTTCCAACACTTTCCCAG
CaCDC4_probe_R	CACTACTAGTTGGTTGCTGT

^aRestriction enzyme sites are in italics.
^bSequences complementary to those encoding 6×His and FLAG are in lower case letters. The italics has been used for restriction enzymes as in note “a”. The underline is new replaced with lower case letters.

ISSN: 1423-0127