Znf179 regulates the expression of Plzf at protein level. COS-1 cells were transfected with EGFP or EGFP-Znf179 together with Flag or Flag-Plzf. ( A ) After 24 h transfection, the expression of Plzf and Znf179 were determined by Western blot analysis. (B) Relative amounts of Plzf protein were quantified and normalized to β-actin. ( C ) Relative amounts of Plzf RNA were determined by quantitative RT-PCR and normalized to β-actin. Values represent the mean ± S.E.M. of at least three independent experiments. Statistical analysis was performed using Student’s t-test: *P < 0.05 versus the control group.